Journal: iScience

Article Title: Identification of proteotoxic and proteoprotective bacteria that non-specifically affect proteins associated with neurodegenerative diseases

doi: 10.1016/j.isci.2024.110828

Figure Lengend Snippet:

Article Snippet: Propionibacterium propionicum , BEI Resources , HM-209.

Techniques: Virus, Recombinant, Membrane, Western Blot, Software

Journal: Microorganisms

Article Title: New Insights into the Physiology of the Propionate Producers Anaerotignum propionicum and Anaerotignum neopropionicum (Formerly Clostridium propionicum and Clostridium neopropionicum ).

doi: 10.3390/microorganisms11030685

Figure Lengend Snippet: Figure 1. Overview of Gram type, cell wall structure, and surface layers of A. propionicum (A–C) and A. neopropionicum (D–F). Pictures were obtained using light microscopy, transmission, and scanning electron microscopy. (A,D) Results of Gram staining; (B,E) results of transmission electron microscopic analyses; (C,F) results of scanning electron microscopic analyses. CM, cytoplasmic membrane; PG, peptidoglycan; SL, surface layer.

Article Snippet: A. propionicum was grown at 37 ◦C in DSMZ medium 156, which contained (per liter) 3 g peptone, 4 g yeast extract, 0.1 g MgSO4 × 7 H2O, 18 mg FeSO4 × 7 H2O, 5 mL potassium phosphate buffer (1 M, pH 7.1), 2.5 mL saturated CaSO4 solution, 1 g NaHCO3, 0.3 g L-cysteine-HCl × H2O, and 1 mg resazurin.

Techniques: Light Microscopy, Transmission Assay, Electron Microscopy, Staining, Membrane

Journal: Microorganisms

Article Title: New Insights into the Physiology of the Propionate Producers Anaerotignum propionicum and Anaerotignum neopropionicum (Formerly Clostridium propionicum and Clostridium neopropionicum ).

doi: 10.3390/microorganisms11030685

Figure Lengend Snippet: Figure 2. Overview of growth experiments conducted with A. propionicum and A. neopropionicum using different carbon sources. (A) Growth (OD600, (left)), substrate consumption (middle), and product formation (right) of A. propionicum; (B) growth (left), substrate consumption (middle), and product formation (right) of A. neopropionicum. Black circles, growth with D-lactate; grey squares, growth with ethanol; blue triangles, growth with L-alanine (A. propionicum) or xylose (A. neopropio- nicum). Error bars represent standard deviations, n = 3.

Article Snippet: A. propionicum was grown at 37 ◦C in DSMZ medium 156, which contained (per liter) 3 g peptone, 4 g yeast extract, 0.1 g MgSO4 × 7 H2O, 18 mg FeSO4 × 7 H2O, 5 mL potassium phosphate buffer (1 M, pH 7.1), 2.5 mL saturated CaSO4 solution, 1 g NaHCO3, 0.3 g L-cysteine-HCl × H2O, and 1 mg resazurin.

Techniques:

Journal: Microorganisms

Article Title: New Insights into the Physiology of the Propionate Producers Anaerotignum propionicum and Anaerotignum neopropionicum (Formerly Clostridium propionicum and Clostridium neopropionicum ).

doi: 10.3390/microorganisms11030685

Figure Lengend Snippet: Figure 3. Growth of A. propionicum (blue) and A. neopropionicum (orange) in presence of 50 mM sugar. Cultures without supplementation of a substrate served as a negative control, addition of 50 mM L-alanine served as a positive control for growth of A. propionicum. Cultivation was performed in 5 mL respective medium with supplemented carbon sources. Starting OD600 was set to 0.1 for A. propionicum and 0.05 for A. neopropionicum. Error bars represent standard deviations, n = 3.

Article Snippet: A. propionicum was grown at 37 ◦C in DSMZ medium 156, which contained (per liter) 3 g peptone, 4 g yeast extract, 0.1 g MgSO4 × 7 H2O, 18 mg FeSO4 × 7 H2O, 5 mL potassium phosphate buffer (1 M, pH 7.1), 2.5 mL saturated CaSO4 solution, 1 g NaHCO3, 0.3 g L-cysteine-HCl × H2O, and 1 mg resazurin.

Techniques: Negative Control, Positive Control